An Enzyme-Based Interdigitated Electrode-Type Biosensor for Detecting Low Concentrations of H₂O₂ Vapor/Aerosol

  • This work introduces a novel method for the detection of H₂O₂ vapor/aerosol of low concentrations, which is mainly applied in the sterilization of equipment in medical industry. Interdigitated electrode (IDE) structures have been fabricated by means of microfabrication techniques. A differential setup of IDEs was prepared, containing an active sensor element (active IDE) and a passive sensor element (passive IDE), where the former was immobilized with an enzymatic membrane of horseradish peroxidase that is selective towards H₂O₂. Changes in the IDEs’ capacitance values (active sensor element versus passive sensor element) under H₂O₂ vapor/aerosol atmosphere proved the detection in the concentration range up to 630 ppm with a fast response time (<60 s). The influence of relative humidity was also tested with regard to the sensor signal, showing no cross-sensitivity. The repeatability assessment of the IDE biosensors confirmed their stable capacitive signal in eight subsequent cycles of exposure to H₂O₂ vapor/aerosol. Room-temperature detection of H₂O₂ vapor/aerosol with such miniaturized biosensors will allow a future three-dimensional, flexible mapping of aseptic chambers and help to evaluate sterilization assurance in medical industry.

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Author:Farnoosh Vahidpour, Yousef AlghazaliORCiD, Sevilay Akca, Gregor Hommes, Michael J. SchöningORCiD
Parent Title (English):Chemosensors
Place of publication:Basel
Document Type:Article
Year of Completion:2022
Date of first Publication:2022/05/26
Date of the Publication (Server):2023/01/25
Length:18 Seiten
This article belongs to the Special Issue "Bioinspired Chemical Sensors and Micro-Nano Devices"
Institutes:FH Aachen / INB - Institut für Nano- und Biotechnologien
collections:Open Access
Verlag / MDPI
Licence (German):License LogoCreative Commons - Namensnennung