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Method for production of single-stranded macronucleotides

  • The invention relates to a method for production of single-stranded macronucleotides by amplifying and ligating an extended monomeric single-stranded target nucleic acid sequence (targetss) into a repetitive cluster of double-stranded target nucleic acid sequences (targetds), and subsequently cloning the construct into a vector (aptagene vector). The aptagene vector is transformed into host cells for replication of the aptagene and isolated in order to optain single-stranded target sequences (targetss). The invention also relates to single-stranded nucleic acids, produced by a method of the invention.

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Author:Ralf Stadtmüller, Nils Tippkötter, Roland Ulber
Document Type:Patent
Year of Completion:2015
Date of the Publication (Server):2020/06/26
Patent auch unter EP2774996, EP2774996, US2017145460 und US9944966 veröffentlicht.
Institutes:FH Aachen / Fachbereich Chemie und Biotechnologie
Patent Number:US2015252399
Country of Patent Application:Weltweit
Patent Year applied:2014