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Effects of spermine NONOate and ATP on the thermal stability of hemoglobin

  • Background Minor changes in protein structure induced by small organic and inorganic molecules can result in significant metabolic effects. The effects can be even more profound if the molecular players are chemically active and present in the cell in considerable amounts. The aim of our study was to investigate effects of a nitric oxide donor (spermine NONOate), ATP and sodium/potassium environment on the dynamics of thermal unfolding of human hemoglobin (Hb). The effect of these molecules was examined by means of circular dichroism spectrometry (CD) in the temperature range between 25°C and 70°C. The alpha-helical content of buffered hemoglobin samples (0.1 mg/ml) was estimated via ellipticity change measurements at a heating rate of 1°C/min. Results Major results were: 1) spermine NONOate persistently decreased the hemoglobin unfolding temperature T u irrespectively of the Na + /K + environment, 2) ATP instead increased the unfolding temperature by 3°C in both sodium-based and potassium-based buffers and 3) mutual effects of ATP and NO were strongly influenced by particular buffer ionic compositions. Moreover, the presence of potassium facilitated a partial unfolding of alpha-helical structures even at room temperature. Conclusion The obtained data might shed more light on molecular mechanisms and biophysics involved in the regulation of protein activity by small solutes in the cell.

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Metadaten
Author:Rasha Bassam, Jürgen Hescheler, Aysegül Temiz ArtmannORCiD, Gerhard ArtmannORCiD, Ilya DigelORCiD
DOI:https://doi.org/10.1186/2046-1682-5-16
ISSN:2046-1682
Parent Title (English):BMC Biophysics
Publisher:BioMed Central
Place of publication:London
Document Type:Article
Language:English
Year of Completion:2012
Date of the Publication (Server):2012/12/18
Tag:Circular Dichroism; NONOate; Nitric Oxide; Nitric Oxide Donor
Volume:5
Length:Art. 16
Link:https://doi.org/10.1186/2046-1682-5-16
Zugriffsart:weltweit
Institutes:FH Aachen / Fachbereich Medizintechnik und Technomathematik
FH Aachen / IfB - Institut für Bioengineering
collections:Verlag / BioMed Central
Licence (German):License LogoCreative Commons - Namensnennung