pH-based detection of phenylalnine by potentiometric and colorimetric methods
- In this paper, methods of sample preparation for potentiometric measurement of phenylalanine are presented. Basing on the spectrophotometric measurements of phenylalanine, the concentrations of reagents of the enzymatic reaction (10 mM L-Phe, 0,4 mM NAD+, 2U L-PheDH) were determined. Then, the absorption spectrum of the reaction product, NADH, was monitored (maximum peak at 340 nm). The results obtained by the spectrophotometric method were compared with the results obtained by the colourimetry, using pH indicators. The above-mentioned two methods will be used as references for potentiometric measurements of phenylalanine concentration.
| Verfasserangaben: | Dorota G. Pijanowska, Elzbieta Remiszewska |
|---|---|
| URN: | urn:nbn:de:hbz:a96-opus-1536 |
| DOI: | https://doi.org/10.21269/123 |
| Dokumentart: | Konferenzveröffentlichung |
| Sprache: | Englisch |
| Erscheinungsjahr: | 2006 |
| Veröffentlichende Institution: | Fachhochschule Aachen |
| Beteiligte Körperschaft: | International Symposium on Sensor Science, I3S 2005 <3; 2005; Juelich, Germany> |
| Datum der Publikation (Server): | 22.05.2006 |
| Freies Schlagwort / Tag: | Phenylalanine determination; enzymatic methods; pH-based biosensing |
| GND-Schlagwort: | Biosensor |
| Quelle: | http://www.mdpi.org/sensors/papers/s6040428.pdf |
| Fachbereiche und Einrichtungen: | FH Aachen / Fachbereich Medizintechnik und Technomathematik |
| DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| collections: | FH Aachen / International Symposium on Sensor Science, I3S 2005 <3; 2005; Ju |
