Gold nanoparticle-modified capacitive field-effect sensors: Studying the surface density of nanoparticles and coupling of charged polyelectrolyte macromolecules
- The coupling of ligand-stabilized gold nanoparticles with field-effect devices offers new possibilities for label-free biosensing. In this work, we study the immobilization of aminooctanethiol-stabilized gold nanoparticles (AuAOTs) on the silicon dioxide surface of a capacitive field-effect sensor. The terminal amino group of the AuAOT is well suited for the functionalization with biomolecules. The attachment of the positively-charged AuAOTs on a capacitive field-effect sensor was detected by direct electrical readout using capacitance-voltage and constant capacitance measurements. With a higher particle density on the sensor surface, the measured signal change was correspondingly more pronounced. The results demonstrate the ability of capacitive field-effect sensors for the non-destructive quantitative validation of nanoparticle immobilization. In addition, the electrostatic binding of the polyanion polystyrene sulfonate to the AuAOT-modified sensor surface was studied as a model system for the label-free detection of charged macromolecules. Most likely, this approach can be transferred to the label-free detection of other charged molecules such as enzymes or antibodies.
Verfasserangaben: | Tobias KarschuckORCiD, Corinna Kaulen, Arshak PoghossianORCiD, Patrick H. Wagner, Michael Josef SchöningORCiD |
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DOI: | https://doi.org/10.1002/elsa.202100179 |
ISSN: | 0938-5193 |
Titel des übergeordneten Werkes (Englisch): | Electrochemical Science Advances |
Verlag: | Wiley-VCH |
Verlagsort: | Weinheim |
Dokumentart: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Erscheinungsjahr: | 2021 |
Datum der Erstveröffentlichung: | 11.11.2021 |
Datum der Publikation (Server): | 26.01.2023 |
Freies Schlagwort / Tag: | capacitive EIS sensor; detection of charged macromolecules; field-effect sensor; gold nanoparticles; polystyrene sulfonate |
Jahrgang: | 2 |
Ausgabe / Heft: | 5 |
Umfang: | 10 Seiten |
Link: | https://doi.org/10.1002/elsa.202100179 |
Zugriffsart: | weltweit |
Fachbereiche und Einrichtungen: | FH Aachen / INB - Institut für Nano- und Biotechnologien |
open_access (DINI-Set): | open_access |
collections: | Verlag / Wiley-VCH |
Open Access / Gold | |
Lizenz (Deutsch): | Creative Commons - Namensnennung |