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Towards a multi-enzyme capacitive field-effect biosensor by comparative study of drop-coating and nano-spotting technique

  • Multi-enzyme immobilization onto a capacitive field-effect biosensor by nano-spotting technique is presented. The nano-spotting technique allows to immobilize different enzymes simultaneously on the sensor surface with high spatial resolution without additional photolithographical patterning. The amount of applied enzymatic cocktail on the sensor surface can be tailored. Capacitive electrolyte-insulator-semiconductor (EIS) field-effect sensors with Ta2O5 as pH-sensitive transducer layer have been chosen to immobilize the three different (pL droplets) enzymes penicillinase, urease, and glucose oxidase. Nano-spotting immobilization is compared to conventional drop-coating method by defining different geometrical layouts on the sensor surface (fully, half-, and quarter-spotted). The drop diameter is varying between 84 µm and 102 µm, depending on the number of applied drops (1 to 4) per spot. For multi-analyte detection, penicillinase and urease are simultaneously nano-spotted on the EIS sensor. Sensor characterization was performed by C/V (capacitance/voltage) and ConCap (constant capacitance) measurements. Average penicillin, glucose, and urea sensitivities for the spotted enzymes were 81.7 mV/dec, 40.5 mV/dec, and 68.9 mV/dec, respectively.

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Metadaten
Author:Denise MolinnusORCiD, Stefan BegingORCiD, Carsten Lowis, Michael Josef SchöningORCiD
DOI:https://doi.org/10.3390/s20174924
ISBN:1424-8220
Parent Title (English):Sensors
Publisher:MDPI
Place of publication:Basel
Document Type:Article
Language:English
Year of Completion:2020
Date of the Publication (Server):2020/10/19
Volume:20
Issue:17 art. no. 4924
Note:
Special issue: Multisensor Systems and Signal Processing in Analytical Chemistry
Link:https://doi.org/10.3390/s20174924
Zugriffsart:weltweit
collections:Verlag / MDPI
Open Access / Gold
Licence (German):License LogoCreative Commons - Namensnennung