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Assessment of dot-blot ELISA sensitivity on membrane sorbent using various peroxidase substrates

  • The sensitivity of the peroxidase reaction in dot-blot ELISA significantly depends on the substrate. The highest sensitivity is observed using benzidine and diamine- phenol combinations as the substrates due to the reaction of the coupled oxidation (NADI)

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Metadaten
Author:A. P. Bogoyavlenskiy, Ilya Digel, V. E. Berezin
DOI:https://doi.org/10.21269/322
Document Type:Article
Language:English
Year of Completion:1997
Publishing Institution:Fachhochschule Aachen
Date of the Publication (Server):2012/10/26
Tag:ELISA; aromatic amines; naphtols; phenols
GND Keyword:Enzyme-linked immunosorbent assay; Peroxidase
Source:Biochemistry (Moscow) ; Bd. 62(1997), H. 8, S. 870 - 871
Institutes:FH Aachen / Fachbereich Medizintechnik und Technomathematik
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit