Surface modification for microreactor fabrication
- In this paper, methods of surface modification of different supports, i.e. glass and polymeric beads for enzyme immobilisation are described. The developed method of enzyme immobilisation is based on Schiff’s base formation between the amino groups on the enzyme surface and the aldehyde groups on the chemically modified surface of the supports. The surface of silicon modified by APTS and GOPS with immobilised enzyme was characterised by atomic force microscopy (AFM), time-of-flight secondary ion mass spectroscopy (ToF-SIMS) and infrared spectroscopy (FTIR). The supports with immobilised enzyme (urease) were also tested in combination with microreactors fabricated in silicon and Perspex, operating in a flow-through system. For microreactors filled with urease immobilised on glass beads (Sigma) and on polymeric beads (PAN), a very high and stable signal (pH change) was obtained. The developed method of urease immobilisation can be stated to be very effective.
Verfasserangaben: | Dorota G. Pijanowska, Elzbieta Remiszewska, Cecilia Pederzolli, Lorenzo Lunelli, Michele Vendano, Roberto Canteri, Konrad Dudzinski, Jerzy Kruk, Wladyslaw Torbicz |
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URN: | urn:nbn:de:hbz:a96-opus-1480 |
DOI: | https://doi.org/10.21269/118 |
Dokumentart: | Konferenzveröffentlichung |
Sprache: | Englisch |
Erscheinungsjahr: | 2006 |
Veröffentlichende Institution: | Fachhochschule Aachen |
Beteiligte Körperschaft: | International Symposium on Sensor Science, I3S 2005 <3; 2005; Juelich, Germany> |
Datum der Publikation (Server): | 22.05.2006 |
Freies Schlagwort / Tag: | Microreactors; enzyme immobilisation; lab-on-a-chip; surface modification |
GND-Schlagwort: | Biosensor |
Quelle: | http://www.mdpi.org/sensors/papers/s6040370.pdf |
Fachbereiche und Einrichtungen: | FH Aachen / Fachbereich Medizintechnik und Technomathematik |
DDC-Klassifikation: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
collections: | FH Aachen / International Symposium on Sensor Science, I3S 2005 <3; 2005; Ju |