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Template bacteria-free fabrication of surface imprinted polymer-based biosensor for E. coli detection using photolithographic mimics: Hacking bacterial adhesion

  • As one class of molecular imprinted polymers (MIPs), surface imprinted polymer (SIP)-based biosensors show great potential in direct whole-bacteria detection. Micro-contact imprinting, that involves stamping the template bacteria immobilized on a substrate into a pre-polymerized polymer matrix, is the most straightforward and prominent method to obtain SIP-based biosensors. However, the major drawbacks of the method arise from the requirement for fresh template bacteria and often non-reproducible bacteria distribution on the stamp substrate. Herein, we developed a positive master stamp containing photolithographic mimics of the template bacteria (E. coli) enabling reproducible fabrication of biomimetic SIP-based biosensors without the need for the “real” bacteria cells. By using atomic force and scanning electron microscopy imaging techniques, respectively, the E. coli-capturing ability of the SIP samples was tested, and compared with non-imprinted polymer (NIP)-based samples and control SIP samples, in which the cavity geometry does not match with E. coli cells. It was revealed that the presence of the biomimetic E. coli imprints with a specifically designed geometry increases the sensor E. coli-capturing ability by an “imprinting factor” of about 3. These findings show the importance of geometry-guided physical recognition in bacterial detection using SIP-based biosensors. In addition, this imprinting strategy was employed to interdigitated electrodes and QCM (quartz crystal microbalance) chips. E. coli detection performance of the sensors was demonstrated with electrochemical impedance spectroscopy (EIS) and QCM measurements with dissipation monitoring technique (QCM-D).

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Metadaten
Author:Dua ÖzsoyluORCiD, Fereshteh Aliazizi, Patrick Wagner, Michael Josef SchöningORCiD
DOI:https://doi.org/10.1016/j.bios.2024.116491
ISSN:1873-4235 (eISSN)
ISSN:0956-5663
Parent Title (English):Biosensors and Bioelectronics
Publisher:Elsevier
Place of publication:Amsterdam
Document Type:Article
Language:English
Year of Completion:2024
Date of the Publication (Server):2024/06/24
Tag:E. coli detection; Master stamp; Photolithographic mimics; Quartz crystal microbalance; Surface imprinted polymer
Volume:261
Article Number:116491
Length:11 Seiten
Note:
Corresponding author: Michael J. Schöning
Link:https://doi.org/10.1016/j.bios.2024.116491
Zugriffsart:weltweit
Institutes:FH Aachen / Fachbereich Medizintechnik und Technomathematik
FH Aachen / INB - Institut für Nano- und Biotechnologien
open_access (DINI-Set):open_access
collections:Verlag / Elsevier
Open Access / Hybrid
Geförderte OA-Publikationen / DEAL Elsevier
Licence (German): Creative Commons - Namensnennung-Nicht kommerziell