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Synthesis of α-hydroxy ketones and vicinal diols with the Bacillus licheniformis DSM 13T butane-2, 3-diol dehydrogenase

  • The enantioselective synthesis of α-hydroxy ketones and vicinal diols is an intriguing field because of the broad applicability of these molecules. Although, butandiol dehydrogenases are known to play a key role in the production of 2,3-butandiol, their potential as biocatalysts is still not well studied. Here, we investigate the biocatalytic properties of the meso-butanediol dehydrogenase from Bacillus licheniformis DSM 13T (BlBDH). The encoding gene was cloned with an N-terminal StrepII-tag and recombinantly overexpressed in E. coli. BlBDH is highly active towards several non-physiological diketones and α-hydroxyketones with varying aliphatic chain lengths or even containing phenyl moieties. By adjusting the reaction parameters in biotransformations the formation of either the α-hydroxyketone intermediate or the diol can be controlled.

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Author:Lukas Muschallik, Carina Ronja Kipp, Inga Recker, Johannes BongaertsORCiD, Martina Pohl, Melanie Gelissen, Michael Josef SchöningORCiD, Thorsten Selmer, Petra SiegertORCiD
Parent Title (English):Journal of Biotechnology
Place of publication:Amsterdam
Document Type:Article
Year of Completion:2020
Date of the Publication (Server):2020/09/29
Issue:Vol. 324
First Page:61
Last Page:70
Institutes:FH Aachen / Fachbereich Chemie und Biotechnologie
FH Aachen / Fachbereich Medizintechnik und Technomathematik
FH Aachen / INB - Institut für Nano- und Biotechnologien
collections:Verlag / Elsevier