The search result changed since you submitted your search request. Documents might be displayed in a different sort order.
  • search hit 1 of 21
Back to Result List

Label-free detection of DNA hybridization with light-addressable potentiometric sensors: comparison of various DNA-immobilization strategies

  • Light-addressable potentiometric sensors (LAPS) consisting of a p-Si-SiO2 and p-Si-SiO2-Au structure, respectively, have been tested for a label-free electrical detection of DNA (deoxyribonucleic acid) hybridization. Three different strategies for immobilizing single-stranded probe DNA (ssDNA) molecules on a LAPS surface have been studied and compared: (a) immobilization of thiol-modified ssDNA on the patterned Au surface via gold-thiol bond, (b) covalent immobilization of amino-modified ssDNA onto the SiO2 surface functionalized with 3-aminopropyltriethoxysilane and (c) layer-by-layer adsorption of negatively charged ssDNA on a positively charged weak polyelectrolyte layer of poly(allylamine hydrochloride).

Export metadata

Additional Services

Share in Twitter Search Google Scholar
Metadaten
Author:Thomas BronderORCiD, Chunsheng WuORCiD, Arshak PoghossianORCiD, Frederik WernerORCiD, M. Keusgen, Michael Josef SchöningORCiD
DOI:https://doi.org/10.1016/j.proeng.2014.11.647
ISSN:1877-7058
Parent Title (English):Procedia Engineering
Publisher:Elsevier
Place of publication:Amsterdam
Document Type:Article
Language:English
Year of Completion:2014
Date of the Publication (Server):2015/01/09
Tag:DNA hybridization; LAPS; field-effect sensor; lable-free detection
Volume:87
First Page:755
Last Page:758
Note:
EUROSENSORS 2014 ; European Conference on Solid-State Transducers <28, 2014>
Link:https://doi.org/10.1016/j.proeng.2014.11.647
Zugriffsart:weltweit
Institutes:FH Aachen / Fachbereich Medizintechnik und Technomathematik
collections:Verlag / Elsevier
Open Access / Gold
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung