Light-Addressable Actuator-Sensor Platform for Monitoring and Manipulation of pH Gradients in Microfluidics: A Case Study with the Enzyme Penicillinase
- The feasibility of light-addressed detection and manipulation of pH gradients inside an electrochemical microfluidic cell was studied. Local pH changes, induced by a light-addressable electrode (LAE), were detected using a light-addressable potentiometric sensor (LAPS) with different measurement modes representing an actuator-sensor system. Biosensor functionality was examined depending on locally induced pH gradients with the help of the model enzyme penicillinase, which had been immobilized in the microfluidic channel. The surface morphology of the LAE and enzyme-functionalized LAPS was studied by scanning electron microscopy. Furthermore, the penicillin sensitivity of the LAPS inside the microfluidic channel was determined with regard to the analyte’s pH influence on the enzymatic reaction rate. In a final experiment, the LAE-controlled pH inhibition of the enzyme activity was monitored by the LAPS.
| Author: | Rene Welden, Melanie Jablonski, Christina Wege, Michael Keusgen, Patrick Hermann Wagner, Torsten WagnerORCiD, Michael Josef SchöningORCiD |
|---|---|
| DOI: | https://doi.org/10.3390/bios11060171 |
| ISSN: | 2079-6374 |
| Parent Title (English): | Biosensors |
| Publisher: | MDPI |
| Place of publication: | Basel |
| Document Type: | Article |
| Language: | English |
| Year of Completion: | 2021 |
| Date of first Publication: | 2021/05/27 |
| Date of the Publication (Server): | 2023/02/17 |
| Tag: | actuator-sensor system; enzyme kinetics; light-addressable electrode; light-addressable potentiometric sensor; microfluidics |
| Volume: | 11 |
| Issue: | 6 |
| Length: | Artikel 171 |
| Note: | This article belongs to the Special Issue "Selected Papers from the 1st International Electronic Conference on Biosensors (IECB 2020)" |
| Link: | https://doi.org/10.3390/bios11060171 |
| Zugriffsart: | weltweit |
| Institutes: | FH Aachen / Fachbereich Chemie und Biotechnologie |
| FH Aachen / INB - Institut für Nano- und Biotechnologien | |
| collections: | Verlag / MDPI |
| Open Access / Gold | |
| Licence (German): |  Creative Commons - Namensnennung |
