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Ureplace: development of a bioreactor for in vitro culturing of cell seeded tubular vessels on collagen scaffolds

  • The demand of replacements for inoperable organs exceeds the amount of available organ transplants. Therefore, tissue engineering developed as a multidisciplinary field of research for autologous in-vitro organs. Such three dimensional tissue constructs request the application of a bioreactor. The UREPLACE bioreactor is used to grow cells on tubular collagen scaffolds OPTIMAIX Sponge 1 with a maximal length of 7 cm, in order to culture in vitro an adequate ureter replacement. With a rotating unit, (urothelial) cells can be placed homogeneously on the inner scaffold surface. Furthermore, a stimulation is combined with this bioreactor resulting in an orientation of muscle cells. These culturing methods request a precise control of several parameters and actuators. A combination of a LabBox and the suitable software LabVision is used to set and conduct parameters like rotation angles, velocities, pressures and other important cell culture values. The bioreactor was tested waterproof successfully. Furthermore, the temperature controlling was adjusted to 37 °C and the CO2 - concentration regulated to 5 %. Additionally, the pH step responses of several substances showed a perfect functioning of the designed flow chamber. All used software was tested and remained stable for several days.

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Author:Volker Seifarth, D. Schehl, Peter Linder, Matthias Gossmann, Ilya Digel, Gerhard Artmann, Dariusz Porst, C. Preiß, Peter Kayser, O. Pack, Aysegül Temiz Artmann
Document Type:Article
Year of Completion:2011
Publishing Institution:Fachhochschule Aachen
Date of the Publication (Server):2012/10/16
GND Keyword:Tissue Engineering; Bioreaktor; Organkultur; Harnleiter
Source:Bulletin of Kazakh National University, biology series ; Bd. 48(2011), H. 2, S. 56 - 59
Institutes:FH Aachen / Fachbereich Medizintechnik und Technomathematik
FH Aachen / IfB - Institut für Bioengineering
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit