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Suspension depletion approach for exemption of infected Solanum jasminoides cells from pospiviroids

  • Despite numerous studies, viroid elimination from infected plants remains a very challenging task. This study introduces for the first time a novel ‘suspension depletion’ approach for exemption of Solanum jasminoides plants from viroids. The proposed method implies initial establishment of suspension cultures of the infected plant cells. The suspended cells were then physically treated (mild thermotherapy, 33 °C), which presumably delayed the replication of the viroid. The viroid concentration in the treated biomass was monitored weekly using pospiviroid-specific PCR. After 10–12 weeks of continuous treatment, a sufficient decrease in viroid concentration was observed such that the infection became undetectable by PCR. The treated single cells then gave rise to microcolonies on a solid culture medium and the obtained viroid-negative clones were further promoted to regenerate into viroid-free plants. Three years of accumulated experimental data suggests feasibility, broad applicability, and good efficacy of the proposed approach.

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Metadaten
Author:Ilya DigelORCiD, V. Wehlitz, Peter Kayser, A. Figiel-Lange, R. Bassam, F. von Rundstedt
DOI:https://doi.org/10.1111/ppa.12750
ISSN:1365-3059
Parent Title (English):Plant Pathology
Publisher:Wiley
Place of publication:Oxford
Document Type:Article
Language:English
Year of Completion:2018
Date of the Publication (Server):2019/11/07
Volume:67
Issue:2
First Page:358
Last Page:365
Link:https://doi.org/10.1111/ppa.12750
Zugriffsart:weltweit
Institutes:FH Aachen / Fachbereich Medizintechnik und Technomathematik
FH Aachen / IfB - Institut für Bioengineering
collections:Verlag / Wiley
Open Access / Hybrid
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell