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3D printed modular immunofiltration columns for frequency mixing-based multiplex magnetic immunodetection

  • For performing point-of-care molecular diagnostics, magnetic immunoassays constitute a promising alternative to established enzyme-linked immunosorbent assays (ELISA) because they are fast, robust and sensitive. Simultaneous detection of multiple biomolecular targets from one body fluid sample is desired. The aim of this work is to show that multiplex magnetic immunodetection based on magnetic frequency mixing by means of modular immunofiltration columns prepared for different targets is feasible. By calculations of the magnetic response signal, the required spacing between the modules was determined. Immunofiltration columns were manufactured by 3D printing and antibody immobilization was performed in a batch approach. It was shown experimentally that two different target molecules in a sample solution could be individually detected in a single assaying step with magnetic measurements of the corresponding immobilization filters. The arrangement order of the filters and of a negative control did not influence the results. Thus, a simple and reliable approach to multi-target magnetic immunodetection was demonstrated.

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Author:Stefan AchtsnichtORCiD, Julia Tödter, Julia Niehues, Matthias Telöken, Andreas Offenhäusser, Hans-Joachim Krause, Florian Schröper
Parent Title (English):Sensors
Place of publication:Basel
Document Type:Article
Year of Completion:2019
Date of the Publication (Server):2023/06/09
Length:15 Seiten
Institutes:FH Aachen / Fachbereich Medizintechnik und Technomathematik
FH Aachen / INB - Institut für Nano- und Biotechnologien
collections:Verlag / MDPI
Licence (German):License LogoCreative Commons - Namensnennung