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Development and characterization of a field-effect biosensor for the detection of acetoin

  • A capacitive electrolyte-insulator-semiconductor (EIS) field-effect biosensor for acetoin detection has been presented for the first time. The EIS sensor consists of a layer structure of Al/p-Si/SiO₂/Ta₂O₅/enzyme acetoin reductase. The enzyme, also referred to as butane-2,3-diol dehydrogenase from B. clausii DSM 8716T, has been recently characterized. The enzyme catalyzes the (R)-specific reduction of racemic acetoin to (R,R)- and meso-butane-2,3-diol, respectively. Two different enzyme immobilization strategies (cross-linking by using glutaraldehyde and adsorption) have been studied. Typical biosensor parameters such as optimal pH working range, sensitivity, hysteresis, linear concentration range and long-term stability have been examined by means of constant-capacitance (ConCap) mode measurements. Furthermore, preliminary experiments have been successfully carried out for the detection of acetoin in diluted white wine samples.

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Author:Denise MolinnusORCiD, Lukas Muschallik, Laura Osorio Gonzalez, Johannes Bongaerts, Torsten WagnerORCiD, Thorsten Selmer, Petra SiegertORCiD, Michael Keusgen, Michael Josef SchöningORCiD
Parent Title (English):Biosensors and Bioelectronics
Place of publication:Amsterdam
Document Type:Article
Year of Completion:2018
Date of the Publication (Server):2018/06/26
First Page:1
Last Page:6
Institutes:FH Aachen / Fachbereich Chemie und Biotechnologie
FH Aachen / Fachbereich Medizintechnik und Technomathematik
FH Aachen / INB - Institut für Nano- und Biotechnologien
collections:Verlag / Elsevier