Capacitive field‐effect biosensor applied for the detection of acetoin in alcoholic beverages and fermentation broths
- An acetoin biosensor based on a capacitive electrolyte–insulator–semiconductor (EIS) structure modified with the enzyme acetoin reductase, also known as butane-2,3-diol dehydrogenase (Bacillus clausii DSM 8716ᵀ), is applied for acetoin detection in beer, red wine, and fermentation broth samples for the first time. The EIS sensor consists of an Al/p-Si/SiO₂/Ta₂O₅ layer structure with immobilized acetoin reductase on top of the Ta₂O₅ transducer layer by means of crosslinking via glutaraldehyde. The unmodified and enzyme-modified sensors are electrochemically characterized by means of leakage current, capacitance–voltage, and constant capacitance methods, respectively.
| Author: | Melanie Jablonski, Felix Münstermann, Jasmina Nork, Denise MolinnusORCiD, Lukas Muschallik, Johannes Bongaerts, Torsten WagnerORCiD, Michael Keusgen, Petra SiegertORCiD, Michael Josef SchöningORCiD |
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| DOI: | https://doi.org/10.1002/pssa.202000765 |
| ISSN: | 1862-6319 |
| Parent Title (English): | physica status solidi (a) applications and materials science |
| Publisher: | Wiley-VCH |
| Place of publication: | Weinheim |
| Document Type: | Article |
| Language: | English |
| Year of Completion: | 2021 |
| Date of the Publication (Server): | 2021/01/25 |
| Tag: | acetoin; acetoin reductase; alcoholic beverages; biosensors; capacitive field-effect sensors |
| Volume: | 218 |
| Issue: | 13 |
| Length: | 7 Seiten |
| Link: | https://doi.org/10.1002/pssa.202000765 |
| Zugriffsart: | weltweit |
| Institutes: | FH Aachen / Fachbereich Chemie und Biotechnologie |
| FH Aachen / Fachbereich Medizintechnik und Technomathematik | |
| FH Aachen / INB - Institut für Nano- und Biotechnologien | |
| collections: | Open Access |
| Verlag / Wiley-VCH | |
| Licence (German): |  Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung |
