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Adsorption of bacterial Lipopolysaccharides and blood plasma proteins on modified carbonized materials

  • Bacterial lipopolysaccharides (endotoxins) show strong biological effects at very low concentrations in human beings and many animals when entering the blood stream. These include affecting structure and function of organs and cells, changing metabolic functions, raising body temperature, triggering the coagulation cascade, modifying hemodynamics and causing septic shock. Because of this toxicity, the removal of even minute amounts is essential for safe parenteral administration of drugs and also for septic shock patients' care. The absence of a general method for endotoxin removal from liquid interfaces urgently requires finding new methods and materials to overcome this gap. Nanostructured carbonized plant parts is a promising material that showed good adsorption properties due to its vast pore network and high surface area. The aim of this study was comparative measurement of endotoxin- and blood proteins-related adsorption rate and adsorption capacity for different carboneous materials produced at different temperatures and under different surface modifications. As a main surface modificator, positively cbarged polymer, polyethileneimine (PEl) was used. Activated carbon materials showed good adsorption properties for LPS and some proteins used in the experiments. During the batch experiments, several techniques (dust removal, autoclaving) were used and optimized for improving the material's adsorption behavior. Also, with the results obtained it was possible to differentiate the materials according to their adsorption capacity and kinetic characteristics. Modification of the surface apparently has not affected hemoglobin binding to the adsorbent's surface. Obtained adsorption isotherms can be used as a powerful tool for designing of future column-based setups for blood purification from LPS, which is especially important for septic shock treatment.

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Author:Azhar A. Zhubanova, S. K. Aknazarov, Zulkhair Mansurov, Ilya Digel, A. A. Kozhalakova, Nuraly Shardarbekovich Akimbekov, Carlos O'Heras, S. Tazhibayeva, Gerhard Artmann, Aysegül Temiz Artmann
Document Type:Article
Year of Completion:2010
Publishing Institution:Fachhochschule Aachen
Date of the Publication (Server):2012/10/18
Tag:Lipopolysaccharide; nanostrukturierte carbonisierte Pflanzenteile
lipopolysaccharides; nanostructured carbonized plant parts
GND Keyword:Kohlenstofffaser
Source:International Journal of Biology and Chemistry ; Bd. 1 (2010), S. 81 - 90
Institutes:FH Aachen / Fachbereich Medizintechnik und Technomathematik
FH Aachen / IfB - Institut für Bioengineering
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit