Electrostatic Detection of Unlabelled Single- and Double-stranded DNA Using Capacitive Field-effect Devices Functionalized with a Positively Charged Polyelectrolyte Layer
- Capacitive field-effect electrolyte-insulator-semiconductor sensors consisting of an Al-p-Si-SiO2 structure have been used for the electrical detection of unlabelled single- and double-stranded DNA (dsDNA) molecules by their intrinsic charge. A simple functionalization protocol based on the layer-by-layer (LbL) technique was used to prepare a weak polyelectrolyte/probe-DNA bilayer, followed by the hybridization with complementary target DNA molecules. Due to the flat orientation of the LbL-adsorbed DNA molecules, a high sensor signal has been achieved. In addition, direct label-free detection of in-solution hybridized dsDNA molecules has been studied.
| Author: | Thomas BronderORCiD, Arshak PoghossianORCiD, S. Scheja, Chunsheng WuORCiD, M. Keusgen, Michael Josef SchöningORCiD |
|---|---|
| DOI: | https://doi.org/10.1016/j.proeng.2015.08.710 |
| ISSN: | 1877-7058 |
| Parent Title (English): | Procedia Engineering |
| Publisher: | Elsevier |
| Place of publication: | Amsterdam |
| Document Type: | Article |
| Language: | English |
| Year of Completion: | 2015 |
| Date of the Publication (Server): | 2015/10/01 |
| Volume: | 120 |
| First Page: | 544 |
| Last Page: | 547 |
| Note: | Eurosensors 2015 |
| Link: | https://doi.org/10.1016/j.proeng.2015.08.710 |
| Zugriffsart: | weltweit |
| Institutes: | FH Aachen / Fachbereich Medizintechnik und Technomathematik |
| FH Aachen / INB - Institut für Nano- und Biotechnologien | |
| collections: | Verlag / Elsevier |
| Licence (German): |  Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung |
