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Label-free Electrostatic Detection of DNA Amplification by PCR Using Capacitive Field-effect Devices

  • A capacitive field-effect EIS (electrolyte-insulator-semiconductor) sensor modified with a positively charged weak polyelectrolyte of poly(allylamine hydrochloride) (PAH)/single-stranded probe DNA (ssDNA) bilayer has been used for a label-free electrostatic detection of pathogen-specific DNA amplification via polymerase chain reaction (PCR). The sensor is able to distinguish between positive and negative PCR solutions, to detect the existence of target DNA amplicons in PCR samples and thus, can be used as tool for a quick verification of DNA amplification and the successful PCR process.

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Metadaten
Author:Arshak PoghossianORCiD, Thomas BronderORCiD, S. Scheja, Chunsheng WuORCiD, C. Metzger-Boddien, M. Keusgen, Michael Josef SchöningORCiD
DOI:https://doi.org/10.1016/j.proeng.2016.11.512
ISSN:1877-7058
Parent Title (English):Procedia Engineering
Publisher:Elsevier
Place of publication:Amsterdam
Document Type:Conference Proceeding
Language:English
Year of Completion:2016
Date of the Publication (Server):2017/01/19
Volume:Vol. 168
First Page:514
Last Page:517
Note:
Proceedings of the 30th anniversary Eurosensors Conference – Eurosensors 2016, 4-7. Sepember 2016, Budapest, Hungary
Link:https://doi.org/10.1016/j.proeng.2016.11.512
Zugriffsart:weltweit
Institutes:FH Aachen / Fachbereich Medizintechnik und Technomathematik
FH Aachen / INB - Institut für Nano- und Biotechnologien
collections:Verlag / Elsevier
Open Access / Gold
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung