Toward multi-analyte bioarray sensors: LAPS-based on-chip determination of a Michaelis–Menten-like kinetics for cell culturing
- The metabolic activity of Chinese hamster ovary (CHO) cells was observed using a light-addressable potentiometric sensor (LAPS). The dependency toward different glucose concentrations (17–200 mM) follows a Michaelis–Menten kinetics trajectory with Kₘ = 32.8 mM, and the obtained Kₘ value in this experiment was compared with that found in literature. In addition, the pH shift induced by glucose metabolism of tumor cells transfected with the HPV-16 genome (C3 cells) was successfully observed. These results indicate the possibility to determine the tumor cells metabolism with a LAPS-based measurement device.
| Author: | Shoko Takenaga, Manfred Biselli, Thomas Schnitzler, Peter Öhlschläger, Torsten WagnerORCiD, Michael Josef SchöningORCiD |
|---|---|
| DOI: | https://doi.org/10.1002/pssa.201330464 |
| ISSN: | 1521-396X (E); 1862-6319 (E-Journal); 0031-8965 (Print); 1862-6300 (Print) |
| Parent Title (English): | Physica status solidi A : Applications and materials science |
| Publisher: | Wiley-VCH |
| Place of publication: | Weinheim |
| Document Type: | Article |
| Language: | English |
| Year of Completion: | 2014 |
| Date of the Publication (Server): | 2014/04/28 |
| Volume: | 211 |
| Issue: | 6 |
| First Page: | 1410 |
| Last Page: | 1415 |
| Link: | https://doi.org/10.1002/pssa.201330464 |
| Zugriffsart: | campus |
| Institutes: | FH Aachen / Fachbereich Chemie und Biotechnologie |
| FH Aachen / INB - Institut für Nano- und Biotechnologien | |
| collections: | Verlag / Wiley-VCH |
