- Bacterial endotoxin (LPS) removal from aqueous solutions is a challenging bioengineering task because of its ubiquity and high physical and chemical stability. Nowadays, adsorption methods of LPS removal are regarded as most advantageous with many different adsorbent materials being developed. In this work, low-cost nanostructured carbonized material (rice husks-CRH) designed by us in accordance for LPS-adsorption. The purpose of the present investigation was to determine the susceptibility of chicken embryos to an injection of endotoxin and CRH-treated endotoxin in vivo during the period of development. Endotoxin extracted from cell walls of E. coli by LPS extraction kid was injected intravenously into fertile eggs, of varying ages. It has been observed that the susceptibility (the development of provisional organs, pyrogenicity, blood analysis and lethal dose (LD50)) of chicken embryos to endotoxin decreased daily as the embryo matured from day 10 to hatching, while CRH-treated LPS had showed weak effect. These data show that the toxin effects of LPS solution was eliminated by CRH, according to the increased resistance of embryos. We consider the obtained results on the basis of novel advanced and economically efficient material CRH are very promising as the increasing number of biotechnological and biomedical applications supposed to be endotoxin-free, as well as still persisting problems with purification of complex mixtures.
