A convenient ninhydrin assay in 96-well format for amino acid-releasing enzymes using an air-stable reagent
- An improved and convenient ninhydrin assay for aminoacylase activity measurements was developed using the commercial EZ Nin™ reagent. Alternative reagents from literature were also evaluated and compared. The addition of DMSO to the reagent enhanced the solubility of Ruhemann's purple (RP). Furthermore, we found that the use of a basic, aqueous buffer enhances stability of RP. An acidic protocol for the quantification of lysine was developed by addition of glacial acetic acid. The assay allows for parallel processing in a 96-well format with measurements microtiter plates.
| Author: | Gerrit HaegerORCiD, Johannes BongaertsORCiD, Petra SiegertORCiD |
|---|---|
| DOI: | https://doi.org/10.1016/j.ab.2022.114819 |
| ISSN: | 1096-0309 |
| Parent Title (English): | Analytical Biochemistry |
| Publisher: | Elsevier |
| Place of publication: | Amsterdam |
| Document Type: | Article |
| Language: | English |
| Year of Completion: | 2022 |
| Date of first Publication: | 2022/07/15 |
| Date of the Publication (Server): | 2023/02/16 |
| Issue: | 624 |
| Length: | Artikel 114819 |
| Link: | https://doi.org/10.1016/j.ab.2022.114819 |
| Zugriffsart: | weltweit |
| Institutes: | FH Aachen / Fachbereich Chemie und Biotechnologie |
| FH Aachen / INB - Institut für Nano- und Biotechnologien | |
| collections: | Verlag / Elsevier |
| Open Access / Hybrid | |
| Licence (German): |  Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung |
