Studying the immobilization of acetoin reductase with Tobacco mosaic virus particles on capacitive field-effect sensors
- A capacitive electrolyte-insulator-semiconductor (EISCAP) biosensor modified with Tobacco mosaic virus (TMV) particles for the detection of acetoin is presented. The enzyme acetoin reductase (AR) was immobilized on the surface of the EISCAP using TMV particles as nanoscaffolds. The study focused on the optimization of the TMV-assisted AR immobilization on the Ta 2 O 5 -gate EISCAP surface. The TMV-assisted acetoin EISCAPs were electrochemically characterized by means of leakage-current, capacitance-voltage, and constant-capacitance measurements. The TMV-modified transducer surface was studied via scanning electron microscopy.
| Author: | Melanie Welden, Robin Severins, Arshak PoghossianORCiD, Christina Wege, Petra SiegertORCiD, Michael Keusgen, Michael Josef SchöningORCiD |
|---|---|
| DOI: | https://doi.org/10.1109/ISOEN54820.2022.9789657 |
| ISBN: | 978-1-6654-5860-3 (Online) |
| ISBN: | 978-1-6654-5861-0 (Print) |
| Parent Title (English): | 2022 IEEE International Symposium on Olfaction and Electronic Nose (ISOEN) |
| Publisher: | IEEE |
| Document Type: | Conference Proceeding |
| Language: | English |
| Year of Completion: | 2022 |
| Date of the Publication (Server): | 2023/02/16 |
| Tag: | Tobacco mosaic virus; acetoin; capacitive field-effect biosensor; enzyme immobilization |
| Length: | 4 Seiten |
| Note: | IEEE International Symposium on Olfaction and Electronic Nose (ISOEN), 29 May 2022 - 01 June 2022, Aveiro, Portugal. |
| Link: | https://doi.org/10.1109/ISOEN54820.2022.9789657 |
| Zugriffsart: | campus |
| Institutes: | FH Aachen / Fachbereich Medizintechnik und Technomathematik |
| FH Aachen / INB - Institut für Nano- und Biotechnologien | |
| collections: | Verlag / IEEE |
