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Real-time monitoring of H₂O₂ sterilization on individual bacillus atrophaeus spores by optical sensing with trapping Raman spectroscopy

  • Hydrogen peroxide (H₂O₂), a strong oxidizer, is a commonly used sterilization agent employed during aseptic food processing and medical applications. To assess the sterilization efficiency with H₂O₂, bacterial spores are common microbial systems due to their remarkable robustness against a wide variety of decontamination strategies. Despite their widespread use, there is, however, only little information about the detailed time-resolved mechanism underlying the oxidative spore death by H₂O₂. In this work, we investigate chemical and morphological changes of individual Bacillus atrophaeus spores undergoing oxidative damage using optical sensing with trapping Raman microscopy in real-time. The time-resolved experiments reveal that spore death involves two distinct phases: (i) an initial phase dominated by the fast release of dipicolinic acid (DPA), a major spore biomarker, which indicates the rupture of the spore’s core; and (ii) the oxidation of the remaining spore material resulting in the subsequent fragmentation of the spores’ coat. Simultaneous observation of the spore morphology by optical microscopy corroborates these mechanisms. The dependence of the onset of DPA release and the time constant of spore fragmentation on H₂O₂ shows that the formation of reactive oxygen species from H₂O₂ is the rate-limiting factor of oxidative spore death.

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Metadaten
Author:Morten Bertz, Denise MolinnusORCiD, Michael Josef SchöningORCiD, Takayuki Homma
DOI:https://doi.org/10.3390/chemosensors11080445
ISSN:2227-9040
Parent Title (English):Chemosensors
Publisher:MDPI
Place of publication:Basel
Document Type:Article
Language:English
Year of Completion:2023
Date of first Publication:2023/08/10
Tag:Bacillus atrophaeus spores; DPA (dipicolinic acid); Raman spectroscopy; optical sensor setup; optical trapping; sterilization
Volume:8
Issue:11
Length:Artikel 445
Note:
This article belongs to the Special Issue "Biosensors and Chemical Sensors for Food and Healthcare Monitoring—Celebrating the 10th Anniversary"
Link:https://doi.org/10.3390/chemosensors11080445
Zugriffsart:weltweit
Institutes:FH Aachen / Fachbereich Medizintechnik und Technomathematik
FH Aachen / INB - Institut für Nano- und Biotechnologien
open_access (DINI-Set):open_access
collections:Verlag / MDPI
Open Access / Gold
Licence (German): Creative Commons - Namensnennung