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Sensitive and rapid detection of cholera toxin subunit B using magnetic frequency mixing detection

  • Cholera is a life-threatening disease caused by the cholera toxin (CT) as produced by some Vibrio cholerae serogroups. In this research we present a method which directly detects the toxin’s B subunit (CTB) in drinking water. For this purpose we performed a magnetic sandwich immunoassay inside a 3D immunofiltration column. We used two different commercially available antibodies to capture CTB and for binding to superparamagnetic beads. ELISA experiments were performed to select the antibody combination. The beads act as labels for the magnetic frequency mixing detection technique. We show that the limit of detection depends on the type of magnetic beads. A nonlinear Hill curve was fitted to the calibration measurements by means of a custom-written python software. We achieved a sensitive and rapid detection of CTB within a broad concentration range from 0.2 ng/ml to more than 700 ng/ml.

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Author:Stefan AchtsnichtORCiD, Christian Neuendorf, Tobias Faßbender, Greta Nölke, Andreas Offenhäusser, Hans-Joachim Krause, Florian Schröper
Parent Title (English):Plos One
Document Type:Article
Year of Completion:2019
Date of the Publication (Server):2023/06/06
Length:15 Seiten
Institutes:FH Aachen / Fachbereich Medizintechnik und Technomathematik
FH Aachen / INB - Institut für Nano- und Biotechnologien
collections:Open Access