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Multiplex detection of different magnetic beads using frequency scanning in magnetic frequency mixing technique

  • In modern bioanalytical methods, it is often desired to detect several targets in one sample within one measurement. Immunological methods including those that use superparamagnetic beads are an important group of techniques for these applications. The goal of this work is to investigate the feasibility of simultaneously detecting different superparamagnetic beads acting as markers using the magnetic frequency mixing technique. The frequency of the magnetic excitation field is scanned while the lower driving frequency is kept constant. Due to the particles’ nonlinear magnetization, mixing frequencies are generated. To record their amplitude and phase information, a direct digitization of the pickup-coil’s signal with subsequent Fast Fourier Transformation is performed. By synchronizing both magnetic beads using frequency scanning in magnetic frequency mixing technique magnetic fields, a stable phase information is gained. In this research, it is shown that the amplitude of the dominant mixing component is proportional to the amount of superparamagnetic beads inside a sample. Additionally, it is shown that the phase does not show this behaviour. Excitation frequency scans of different bead types were performed, showing different phases, without correlation to their diverse amplitudes. Two commercially available beads were selected and a determination of their amount in a mixture is performed as a demonstration for multiplex measurements.

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Author:Stefan AchtsnichtORCiD, Ali Mohammad Pourshahidi, Andreas Offenhäusser, Hans-Joachim Krause
Parent Title (English):Sensors
Place of publication:Basel
Document Type:Article
Year of Completion:2019
Date of the Publication (Server):2023/06/09
Tag:frequency mixing magnetic detection; magnetic sandwich immunoassay; multiparametric immunoassays
Length:13 Seiten
Institutes:FH Aachen / Fachbereich Medizintechnik und Technomathematik
FH Aachen / INB - Institut für Nano- und Biotechnologien
collections:Open Access
Verlag / MDPI
Licence (German):License LogoCreative Commons - Namensnennung