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Enhancement of succinic acid production by Actinobacillus succinogenes in an electro-bioreactor

  • This work examines the electrochemically enhanced production of succinic acid using the bacterium Actinobacillus succinogenes. The principal objective is to enhance the metabolic potential of glucose and CO2 utilization via the C4 pathway in order to synthesize succinic acid. We report on the development of an electro-bioreactor system to increase succinic acid production in a power-2-X approach. The use of activated carbon fibers as electrode surfaces and contact areas allows A. succinogenes to self-initiate biofilm formation. The integration of an electrical potential into the system shifts the redox balance from NAD+ to NADH, increasing the efficiency of metabolic processes. Mediators such as neutral red facilitate electron transfer within the system and optimize the redox reactions that are crucial for increased succinic acid production. Furthermore, the role of carbon nanotubes (CNTs) in electron transfer was investigated. The electro-bioreactor system developed here was operated in batch mode for 48 h and showed improvements in succinic acid yield and concentration. In particular, a run with 100 µM neutral red and a voltage of −600 mV achieved a yield of 0.7 gsuccinate·gglucose−1. In the absence of neutral red, a higher yield of 0.72 gsuccinate·gglucose−1 was achieved, which represents an increase of 14% compared to the control. When a potential of −600 mV was used in conjunction with 500 µg∙L−1 CNTs, a 21% increase in succinate concentration was observed after 48 h. An increase of 33% was achieved in the same batch by increasing the stirring speed. These results underscore the potential of the electro-bioreactor system to markedly enhance succinic acid production.

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Metadaten
Author:Julian TixORCiD, Leon Gotthardt, Joshua Bode, Burak Karabacak, Janne Nordmann, Jan-Niklas Hengsbach, Roland Ulber, Nils TippkötterORCiD
DOI:https://doi.org/10.3390/fermentation10100504
ISSN:2311-5637
Parent Title (English):Fermentation
Publisher:MDPI
Place of publication:Basel
Document Type:Article
Language:English
Year of Completion:2024
Date of first Publication:2024/09/30
Date of the Publication (Server):2024/10/01
Tag:A. succinogenes; electro-bioreactor; electrofermentation; power-to-X; succinate
Volume:10
Issue:10
Article Number:504
Note:
Corresponding author: Nils Tippkötter
Note:
This article belongs to the Special Issue "Advance in Microbial Electrochemical Technologies"
Peer Review:Ja
Link:https://doi.org/10.3390/fermentation10100504
Zugriffsart:weltweit
Institutes:FH Aachen / Fachbereich Chemie und Biotechnologie
collections:Open Access
Verlag / MDPI
Open Access / Gold
Licence (German): Creative Commons - Namensnennung