Influence of temperature, light, and H₂O₂ concentration on microbial spore inactivation: in-situ Raman spectroscopy combined with optical trapping
- To gain insight on chemical sterilization processes, the influence of temperature (up to 70 °C), intense green light, and hydrogen peroxide (H₂O₂) concentration (up to 30% in aqueous solution) on microbial spore inactivation is evaluated by in-situ Raman spectroscopy with an optical trap. Bacillus atrophaeus is utilized as a model organism. Individual spores are isolated and their chemical makeup is monitored under dynamically changing conditions (temperature, light, and H₂O₂ concentration) to mimic industrially relevant process parameters for sterilization in the field of aseptic food processing. While isolated spores in water are highly stable, even at elevated temperatures of 70 °C, exposure to H₂O₂ leads to a loss of spore integrity characterized by the release of the key spore biomarker dipicolinic acid (DPA) in a concentration-dependent manner, which indicates damage to the inner membrane of the spore. Intensive light or heat, both of which accelerate the decomposition of H₂O₂ into reactive oxygen species (ROS), drastically shorten the spore lifetime, suggesting the formation of ROS as a rate-limiting step during sterilization. It is concluded that Raman spectroscopy can deliver mechanistic insight into the mode of action of H₂O₂-based sterilization and reveal the individual contributions of different sterilization methods acting in tandem.
Verfasserangaben: | Morten Bertz, Michael Josef SchöningORCiD, Denise MolinnusORCiD, Takayuki Homma |
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DOI: | https://doi.org/10.1002/pssa.202300866 |
ISSN: | 1862-6319 (Online) |
ISSN: | 1862-6300 (Print) |
Titel des übergeordneten Werkes (Englisch): | Physica status solidi (a) applications and materials science |
Verlag: | Wiley-VCH |
Verlagsort: | Berlin |
Dokumentart: | Wissenschaftlicher Artikel |
Sprache: | Englisch |
Erscheinungsjahr: | 2024 |
Datum der Publikation (Server): | 04.04.2024 |
Freies Schlagwort / Tag: | Raman spectroscopy; hydrogen peroxide; optical spore trapping; sterilization conditions; temperature |
Ausgabe / Heft: | Early View |
Umfang: | 8 Seiten |
Bemerkung: | Corresponding author: Michael J. Schöning |
Link: | https://doi.org/10.1002/pssa.202300866 |
Zugriffsart: | weltweit |
Fachbereiche und Einrichtungen: | FH Aachen / Fachbereich Medizintechnik und Technomathematik |
FH Aachen / INB - Institut für Nano- und Biotechnologien | |
open_access (DINI-Set): | open_access |
collections: | Verlag / Wiley-VCH |
Open Access / Hybrid | |
Geförderte OA-Publikationen / DEAL Wiley | |
Lizenz (Deutsch): | Creative Commons - Namensnennung-Nicht kommerziell |