Enzymatic hydrolysis of beech wood lignocellulose at high solid contents and its utilization as substrate for the production of biobutanol and dicarboxylic acids
- The development of a cost-effective hydrolysis for crude cellulose is an essential part of biorefinery developments. To establish such high solid hydrolysis, a new solid state reactor with static mixing is used. However, concentrations >10% (w/w) cause a rate and yield reduction of enzymatic hydrolysis. By optimizing the synergetic activity of cellulolytic enzymes at solid concentrations of 9%, 17% and 23% (w/w) of crude Organosolv cellulose, glucose concentrations of 57, 113 and 152 g L⁻¹ are reached. However, the glucose yield decreases from 0.81 to 0.72gg⁻¹ at 17% (w/w). Optimal conditions for hydrolysis scale-up under minimal enzyme addition are identified. As result, at 23% (w/w) crude cellulose the glucose yield increases from 0.29 to 0.49gg⁻¹. As proof of its applicability, biobutanol, succinic and itaconic acid are produced with the crude hydrolysate. The potential of the substrate is proven e.g. by a high butanol yield of 0.33gg⁻¹.
Author: | Nils TippkötterORCiD, Anna-Maria Duwe, Sebastian Wiesen, Tim Sieker, Roland UlberORCiD |
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DOI: | https://doi.org/10.1016/j.biortech.2014.06.052 |
Parent Title (English): | Bioresource Technology |
Publisher: | Elsevier |
Place of publication: | Amsterdam |
Document Type: | Article |
Language: | English |
Year of Completion: | 2014 |
Date of the Publication (Server): | 2017/08/18 |
Volume: | 167 |
First Page: | 447 |
Last Page: | 455 |
Link: | https://doi.org/10.1016/j.biortech.2014.06.052 |
Zugriffsart: | campus |
Institutes: | FH Aachen / Fachbereich Chemie und Biotechnologie |
collections: | Verlag / Elsevier |