• search hit 88 of 0
Back to Result List

Light-addressable potentiometric sensor (LAPS) combined with magnetic beads for pharmaceutical screening

  • The light-addressable potentiometric sensor (LAPS) has the unique feature to address different regions of a sensor surface without the need of complex structures. Measurements at different locations on the sensor surface can be performed in a common analyte solution, which distinctly simplifies the fluidic set-up. However, the measurement in a single analyte chamber prevents the application of different drugs or different concentrations of a drug to each measurement spot at the same time as in the case of multi-reservoir-based set-ups. In this work, the authors designed a LAPS-based set-up for cell culture screening that utilises magnetic beads loaded with the endotoxin (lipopolysaccharides, LPS), to generate a spatially distributed gradient of analyte concentration. Different external magnetic fields can be adjusted to move the magnetic beads loaded with a specific drug within the measurement cell. By recording the metabolic activities of a cell layer cultured on top of the LAPS surface, this work shows the possibility to apply different concentrations of a sample along the LAPS measurement spots within a common analyte solution.

Export metadata

Additional Services

Share in Twitter Search Google Scholar
Metadaten
Author:Torsten WagnerORCiD, Wolfgang Vornholt, Frederik WernerORCiD, Tatsuo Yoshinobu, Ko-Ichiro Miyamoto, Michael Keusgen, Michael Josef SchöningORCiD
DOI:https://doi.org/10.1016/j.phmed.2016.03.001
ISSN:2352-4510
Parent Title (English):Physics in medicine
Document Type:Article
Language:English
Year of Completion:2016
Date of the Publication (Server):2016/07/06
Volume:2016
Issue:1
First Page:2
Last Page:7
Link:https://doi.org/10.1016/j.phmed.2016.03.001
Zugriffsart:weltweit
Institutes:FH Aachen / Fachbereich Medizintechnik und Technomathematik
FH Aachen / INB - Institut für Nano- und Biotechnologien
collections:Verlag / Elsevier
Open Access / Gold
Licence (German):License LogoCreative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung