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pH-based detection of phenylalnine by potentiometric and colorimetric methods

  • In this paper, methods of sample preparation for potentiometric measurement of phenylalanine are presented. Basing on the spectrophotometric measurements of phenylalanine, the concentrations of reagents of the enzymatic reaction (10 mM L-Phe, 0,4 mM NAD+, 2U L-PheDH) were determined. Then, the absorption spectrum of the reaction product, NADH, was monitored (maximum peak at 340 nm). The results obtained by the spectrophotometric method were compared with the results obtained by the colourimetry, using pH indicators. The above-mentioned two methods will be used as references for potentiometric measurements of phenylalanine concentration.

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Metadaten
Author:Dorota G. Pijanowska, Elzbieta Remiszewska
URN:urn:nbn:de:hbz:a96-opus-1536
DOI:https://doi.org/10.21269/123
Document Type:Conference Proceeding
Language:English
Year of Completion:2006
Publishing Institution:Fachhochschule Aachen
Contributing Corporation:International Symposium on Sensor Science, I3S 2005 <3; 2005; Juelich, Germany>
Date of the Publication (Server):2006/05/22
Tag:Phenylalanine determination; enzymatic methods; pH-based biosensing
GND Keyword:Biosensor
Source:http://www.mdpi.org/sensors/papers/s6040428.pdf
Institutes:FH Aachen / Fachbereich Medizintechnik und Technomathematik
Dewey Decimal Classification:5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie
collections:FH Aachen / International Symposium on Sensor Science, I3S 2005 <3; 2005; Ju