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Optimization of the immobilization of bacterial spores on glass substrates with organosilanes

  • Spores can be immobilized on biosensors to function as sensitive recognition elements. However, the immobilization can affect the sensitivity and reproducibility of the sensor signal. In this work, three different immobilization strategies with organosilanes were optimized and characterized to immobilize Bacillus atrophaeus spores on glass substrates. Five different silanization parameters were investigated: nature of the solvent, concentration of the silane, silanization time, curing process, and silanization temperature. The resulting silane layers were resistant to a buffer solution (e.g., Ringer solution) with a polysorbate (e.g., Tween®80) and sonication.

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Metadaten
Author:Julio Arreola, Malte Mätzkow, Marlena Palomar Durán, Anton Greeff, Michael Keusgen, Michael Josef SchöningORCiD
DOI:https://doi.org/10.1002/pssa.201532914
ISSN:1862-6319
Parent Title (English):Physica status solidi (A) : Applications and materials science
Publisher:Wiley-VCH
Place of publication:Weinheim
Document Type:Article
Language:English
Year of Completion:2016
Date of the Publication (Server):2016/03/31
Tag:Bacillus atrophaeus; biosensors; endospores; immobilization; organosilanes; silanization
Volume:213
Issue:6
First Page:1463
Last Page:1470
Link:https://doi.org/10.1002/pssa.201532914
Zugriffsart:campus
Institutes:FH Aachen / Fachbereich Medizintechnik und Technomathematik
FH Aachen / INB - Institut für Nano- und Biotechnologien
collections:Verlag / Wiley-VCH