The search result changed since you submitted your search request. Documents might be displayed in a different sort order.
  • search hit 71 of 81
Back to Result List

Functional Toxicology and Pharmacology Test of Cell Induced Mechanical Tensile Stress in 2D and 3D Tissue Cultures

  • Mechanical forces/tensile stresses are critical determinants of cellular growth, differentiation and migration patterns in health and disease. The innovative “CellDrum technology” was designed for measuring mechanical tensile stress of cultured cell monolayers/thin tissue constructs routinely. These are cultivated on very thin silicone membranes in the so-called CellDrum. The cell layers adhere firmly to the membrane and thus transmit the cell forces generated. A CellDrum consists of a cylinder which is sealed from below with a 4 μm thick, biocompatible, functionalized silicone membrane. The weight of cell culture medium bulbs the membrane out downwards. Membrane indentation is measured. When cells contract due to drug action, membrane, cells and medium are lifted upwards. The induced indentation changes allow for lateral drug induced mechanical tension quantification of the micro-tissues. With hiPS-induced (human) Cardiomyocytes (CM) the CellDrum opens new perspectives of individualized cardiac drug testing. Here, monolayers of self-beating hiPS-CMs were grown in CellDrums. Rhythmic contractions of the hiPS-cells induce membrane up-and-down deflections. The recorded cycles allow for single beat amplitude, single beat duration, integration of the single beat amplitude over the beat time and frequency analysis. Dose effects of agonists and antagonists acting on Ca2+ channels were sensitively and highly reproducibly observed. Data were consistent with published reference data as far as they were available. The combination of the CellDrum technology with hiPS-Cardiomyocytes offers a fast, facile and precise system for pharmacological and toxicological studies. It allows new preclinical basic as well as applied research in pharmacolgy and toxicology.

Export metadata

Additional Services

Share in Twitter Search Google Scholar
Metadaten
Author:Gerhard ArtmannORCiD, Haritha Meruvu, Sefa Kizildag, Aysegül Temiz ArtmannORCiD
DOI:https://doi.org/10.1007/978-981-10-7904-7_7
ISBN:978-981-10-7904-7
Parent Title (English):Biological, Physical and Technical Basics of Cell Engineering
Publisher:Springer
Place of publication:Singapore
Editor:Gerhard Artmann, Aysegül Temiz Artmann, Azhar A. Zhubanova, Ilya Digel
Document Type:Part of a Book
Language:English
Year of Completion:2018
Date of the Publication (Server):2018/04/26
First Page:157
Last Page:192
Link:http://doi.org/10.1007/978-981-10-7904-7_7
Institutes:FH Aachen / Fachbereich Chemie und Biotechnologie
FH Aachen / IfB - Institut für Bioengineering
collections:Verlag / Springer