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Development of a temperature‐pulse enhanced electrochemical glucose biosensor and characterization of its stability via scanning electrochemical microscopy

  • Glucose oxidase (GOx) is an enzyme frequently used in glucose biosensors. As increased temperatures can enhance the performance of electrochemical sensors, we investigated the impact of temperature pulses on GOx that was drop-coated on flattened Pt microwires. The wires were heated by an alternating current. The sensitivity towards glucose and the temperature stability of GOx was investigated by amperometry. An up to 22-fold increase of sensitivity was observed. Spatially resolved enzyme activity changes were investigated via scanning electrochemical microscopy. The application of short (<100 ms) heat pulses was associated with less thermal inactivation of the immobilized GOx than long-term heating.

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Metadaten
Author:Stefan Wert, Heiko IkenORCiD, Michael Josef SchöningORCiD, Frank-Michael Matysik
DOI:https://doi.org/10.1002/elan.202100089
ISSN:1521-4109
Parent Title (English):Electroanalysis
Publisher:Wiley-VCH
Place of publication:Weinheim
Document Type:Article
Language:English
Year of Completion:2021
Date of the Publication (Server):2021/04/13
Issue:Early View
Link:https://doi.org/10.1002/elan.202100089
Zugriffsart:weltweit
Institutes:FH Aachen / Fachbereich Medizintechnik und Technomathematik
FH Aachen / INB - Institut für Nano- und Biotechnologien
collections:Verlag / Wiley-VCH
Open Access / Hybrid
Licence (German):License LogoCreative Commons - Namensnennung