pH-based detection of phenylalnine by potentiometric and colorimetric methods
- In this paper, methods of sample preparation for potentiometric measurement of phenylalanine are presented. Basing on the spectrophotometric measurements of phenylalanine, the concentrations of reagents of the enzymatic reaction (10 mM L-Phe, 0,4 mM NAD+, 2U L-PheDH) were determined. Then, the absorption spectrum of the reaction product, NADH, was monitored (maximum peak at 340 nm). The results obtained by the spectrophotometric method were compared with the results obtained by the colourimetry, using pH indicators. The above-mentioned two methods will be used as references for potentiometric measurements of phenylalanine concentration.
| Author: | Dorota G. Pijanowska, Elzbieta Remiszewska |
|---|---|
| URN: | urn:nbn:de:hbz:a96-opus-1536 |
| DOI: | https://doi.org/10.21269/123 |
| Document Type: | Conference Proceeding |
| Language: | English |
| Year of Completion: | 2006 |
| Publishing Institution: | Fachhochschule Aachen |
| Contributing Corporation: | International Symposium on Sensor Science, I3S 2005 <3; 2005; Juelich, Germany> |
| Tag: | Phenylalanine determination; enzymatic methods; pH-based biosensing |
| GND Keyword: | Biosensor |
| Source: | http://www.mdpi.org/sensors/papers/s6040428.pdf |
| Institutes: | FH Aachen / Fachbereich Medizintechnik und Technomathematik |
| Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
| collections: | FH Aachen / International Symposium on Sensor Science, I3S 2005 <3; 2005; Ju |
