Biosensor for direct determination of fenitrothion and EPN using recombinant Pseudomonas putida JS444 with surface expressed organophosphorus hydrolase. 1. modified clark oxygen electrode
- This paper reports a first microbial biosensor for rapid and cost-effective determination of organophosphorus pesticides fenitrothion and EPN. The biosensor consisted of recombinant PNP-degrading/oxidizing bacteria Pseudomonas putida JS444 anchoring and displaying organophosphorus hydrolase (OPH) on its cell surface as biological sensing element and a dissolved oxygen electrode as the transducer. Surfaceexpressed OPH catalyzed the hydrolysis of fenitrothion and EPN to release 3-methyl-4-nitrophenol and p-nitrophenol, respectively, which were oxidized by the enzymatic machinery of Pseudomonas putida JS444 to carbon dioxide while consuming oxygen, which was measured and correlated to the concentration of organophosphates. Under the optimum operating conditions, the biosensor was able to measure as low as 277 ppb of fenitrothion and 1.6 ppm of EPN without interference from phenolic compounds and other commonly used pesticides such as carbamate pesticides, triazine herbicides and organophosphate pesticides without nitrophenyl substituent. The applicability of the biosensor to lake water was also demonstrated.
Author: | Yu Lei, Priti Mulchandani, Wilfred Chen, Ashok Mulchandani |
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URN: | urn:nbn:de:hbz:a96-opus-1573 |
DOI: | https://doi.org/10.21269/127 |
Document Type: | Conference Proceeding |
Language: | English |
Year of Completion: | 2006 |
Publishing Institution: | Fachhochschule Aachen |
Contributing Corporation: | International Symposium on Sensor Science, I3S 2005 <3; 2005; Juelich, Germany> |
Date of the Publication (Server): | 2006/05/22 |
Tag: | EPN; Organophosphorus; Pseudomonas putida; biosensor; fenitrothion |
GND Keyword: | Biosensor |
Source: | http://www.mdpi.org/sensors/papers/s6040466.pdf |
Institutes: | FH Aachen / Fachbereich Medizintechnik und Technomathematik |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
collections: | FH Aachen / International Symposium on Sensor Science, I3S 2005 <3; 2005; Ju |