Assessment of dot-blot ELISA sensitivity on membrane sorbent using various peroxidase substrates
- The sensitivity of the peroxidase reaction in dot-blot ELISA significantly depends on the substrate. The highest sensitivity is observed using benzidine and diamine- phenol combinations as the substrates due to the reaction of the coupled oxidation (NADI)
Author: | Andrey P. Bogoyavlenskiy, Ilya DigelORCiD, Vladimir E. Berezin |
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DOI: | https://doi.org/10.21269/322 |
Document Type: | Article |
Language: | English |
Year of Completion: | 1997 |
Publishing Institution: | Fachhochschule Aachen |
Tag: | ELISA; aromatic amines; naphtols; phenols |
GND Keyword: | Enzyme-linked immunosorbent assay; Peroxidase |
Source: | Biochemistry (Moscow) ; Bd. 62(1997), H. 8, S. 870 - 871 |
Institutes: | FH Aachen / Fachbereich Medizintechnik und Technomathematik |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |