Label-free detection of DNA hybridization with light-addressable potentiometric sensors: comparison of various DNA-immobilization strategies
- Light-addressable potentiometric sensors (LAPS) consisting of a p-Si-SiO2 and p-Si-SiO2-Au structure, respectively, have been tested for a label-free electrical detection of DNA (deoxyribonucleic acid) hybridization. Three different strategies for immobilizing single-stranded probe DNA (ssDNA) molecules on a LAPS surface have been studied and compared: (a) immobilization of thiol-modified ssDNA on the patterned Au surface via gold-thiol bond, (b) covalent immobilization of amino-modified ssDNA onto the SiO2 surface functionalized with 3-aminopropyltriethoxysilane and (c) layer-by-layer adsorption of negatively charged ssDNA on a positively charged weak polyelectrolyte layer of poly(allylamine hydrochloride).
Author: | Thomas BronderORCiD, Chunsheng WuORCiD, Arshak PoghossianORCiD, Frederik WernerORCiD, M. Keusgen, Michael Josef SchöningORCiD |
---|---|
DOI: | https://doi.org/10.1016/j.proeng.2014.11.647 |
ISSN: | 1877-7058 |
Parent Title (English): | Procedia Engineering |
Publisher: | Elsevier |
Place of publication: | Amsterdam |
Document Type: | Article |
Language: | English |
Year of Completion: | 2014 |
Tag: | DNA hybridization; LAPS; field-effect sensor; lable-free detection |
Volume: | 87 |
First Page: | 755 |
Last Page: | 758 |
Note: | EUROSENSORS 2014 ; European Conference on Solid-State Transducers <28, 2014> |
Link: | https://doi.org/10.1016/j.proeng.2014.11.647 |
Zugriffsart: | weltweit |
Institutes: | FH Aachen / Fachbereich Medizintechnik und Technomathematik |
collections: | Verlag / Elsevier |
Open Access / Gold | |
Licence (German): | ![]() |