Optimization of the immobilization of bacterial spores on glass substrates with organosilanes
- Spores can be immobilized on biosensors to function as sensitive recognition elements. However, the immobilization can affect the sensitivity and reproducibility of the sensor signal. In this work, three different immobilization strategies with organosilanes were optimized and characterized to immobilize Bacillus atrophaeus spores on glass substrates. Five different silanization parameters were investigated: nature of the solvent, concentration of the silane, silanization time, curing process, and silanization temperature. The resulting silane layers were resistant to a buffer solution (e.g., Ringer solution) with a polysorbate (e.g., Tween®80) and sonication.
| Author: | Julio Arreola, Malte Mätzkow, Marlena Palomar Durán, Anton Greeff, Michael Keusgen, Michael Josef SchöningORCiD |
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| DOI: | https://doi.org/10.1002/pssa.201532914 |
| ISSN: | 1862-6319 |
| Parent Title (English): | Physica status solidi (A) : Applications and materials science |
| Publisher: | Wiley-VCH |
| Place of publication: | Weinheim |
| Document Type: | Article |
| Language: | English |
| Year of Completion: | 2016 |
| Tag: | Bacillus atrophaeus; biosensors; endospores; immobilization; organosilanes; silanization |
| Volume: | 213 |
| Issue: | 6 |
| First Page: | 1463 |
| Last Page: | 1470 |
| Link: | https://doi.org/10.1002/pssa.201532914 |
| Zugriffsart: | campus |
| Institutes: | FH Aachen / Fachbereich Medizintechnik und Technomathematik |
| FH Aachen / INB - Institut für Nano- und Biotechnologien | |
| collections: | Verlag / Wiley-VCH |
