Label-free Electrostatic Detection of DNA Amplification by PCR Using Capacitive Field-effect Devices
- A capacitive field-effect EIS (electrolyte-insulator-semiconductor) sensor modified with a positively charged weak polyelectrolyte of poly(allylamine hydrochloride) (PAH)/single-stranded probe DNA (ssDNA) bilayer has been used for a label-free electrostatic detection of pathogen-specific DNA amplification via polymerase chain reaction (PCR). The sensor is able to distinguish between positive and negative PCR solutions, to detect the existence of target DNA amplicons in PCR samples and thus, can be used as tool for a quick verification of DNA amplification and the successful PCR process.
| Author: | Arshak PoghossianORCiD, Thomas BronderORCiD, S. Scheja, Chunsheng WuORCiD, C. Metzger-Boddien, M. Keusgen, Michael Josef SchöningORCiD |
|---|---|
| DOI: | https://doi.org/10.1016/j.proeng.2016.11.512 |
| ISSN: | 1877-7058 |
| Parent Title (English): | Procedia Engineering |
| Publisher: | Elsevier |
| Place of publication: | Amsterdam |
| Document Type: | Conference Proceeding |
| Language: | English |
| Year of Completion: | 2016 |
| Volume: | Vol. 168 |
| First Page: | 514 |
| Last Page: | 517 |
| Note: | Proceedings of the 30th anniversary Eurosensors Conference – Eurosensors 2016, 4-7. Sepember 2016, Budapest, Hungary |
| Link: | https://doi.org/10.1016/j.proeng.2016.11.512 |
| Zugriffsart: | weltweit |
| Institutes: | FH Aachen / Fachbereich Medizintechnik und Technomathematik |
| FH Aachen / INB - Institut für Nano- und Biotechnologien | |
| open_access (DINI-Set): | open_access |
| collections: | Verlag / Elsevier |
| Open Access / Gold | |
| Licence (German): |  Creative Commons - Namensnennung-Nicht kommerziell-Keine Bearbeitung |
