Label-free detection of double-stranded DNA molecules with polyelectrolyte-modified capacitive field-effect sensors

Markierungsfreie Detektion doppelsträngiger DNA Moleküle mit Hilfe von Polyelektrolyt-modifizierten kapazitiven Feldeffekt-Sensoren

  • In this study, polyelectrolyte-modified field-effect-based electrolyte-insulator-semiconductor (EIS) devices have been used for the label-free electrical detection of double-stranded deoxyribonucleic acid (dsDNA)molecules. The sensor-chip functionalization with a positively charged polyelectrolyte layer provides the possibility of direct adsorptive binding of negatively charged target DNA oligonucleotides onto theSiO2-chip surface.EIS sensors can be utilized as a tool to detect surface-charge changes; the electrostatic adsorption of oligonucleotides onto the polyelectrolyte layer leads to a measureable surface-potential change. Signals of 39mV have been recorded after the incubation with the oligonucleotide solution. Besides the electrochemical experiments, the successful adsorption of dsDNA onto the polyelectrolyte layer has been verified via fluorescence microscopy. The presented results demonstrate that the signal recording of EISchips, which are modified with a polyelectrolyte layer, canbe used as a favorable approach for a fast, cheap and simple detection method for dsDNA.

Export metadata

Additional Services

Share in X Search Google Scholar
Metadaten
Author:Thomas BronderORCiD, Arshak PoghossianORCiD, Michael Keusgen, Michael Josef SchöningORCiD
DOI:https://doi.org/10.1515/teme-2017-0015
Parent Title (English):tm - Technisches Messen
Publisher:De Gruyter
Place of publication:Oldenbourg
Document Type:Article
Language:English
Year of Completion:2017
Volume:84
Issue:10
First Page:628
Last Page:634
Link:https://doi.org/10.1515/teme-2017-0015
Zugriffsart:campus
Institutes:FH Aachen / Fachbereich Medizintechnik und Technomathematik
FH Aachen / INB - Institut für Nano- und Biotechnologien
collections:Verlag / De Gruyter