Albumin-lysozyme interactions: cooperative adsorption on titanium and enzymatic activity
- The interplay of albumin (BSA) and lysozyme (LYZ) adsorbed simultaneously on titanium was analyzed by gel electrophoresis and BCA assay. It was found that BSA and lysozyme adsorb cooperatively. Additionally, the isoelectric point of the respective protein influences the adsorption. Also, the enzymatic activity of lysozyme and amylase (AMY) in mixtures with BSA was considered with respect to a possible influence of protein-protein interaction on enzyme activity. Indeed, an increase of lysozyme activity in the presence of BSA could be observed. In contrast, BSA does not influence the activity of amylase.
| Author: | C. Rösch, F. Kratz, T. Hering, S. Trautmann, N. Umanskaya, Nils TippkötterORCiD, C.M. Müller-Renno, Roland UlberORCiD, M. Hannig, C. Ziegler |
|---|---|
| DOI: | https://doi.org/10.1016/j.colsurfb.2016.09.048 |
| Parent Title (English): | Colloids and Surfaces B: Biointerfaces |
| Publisher: | Elsevier |
| Place of publication: | Amsterdam |
| Document Type: | Article |
| Language: | English |
| Year of Completion: | 2016 |
| Volume: | 149 |
| Issue: | 1 |
| First Page: | 115 |
| Last Page: | 121 |
| Link: | https://doi.org/10.1016/j.colsurfb.2016.09.048 |
| Zugriffsart: | campus |
| Institutes: | FH Aachen / Fachbereich Chemie und Biotechnologie |
| collections: | Verlag / Elsevier |
