Enzymatic hydrolysis of beech wood lignocellulose at high solid contents and its utilization as substrate for the production of biobutanol and dicarboxylic acids

  • The development of a cost-effective hydrolysis for crude cellulose is an essential part of biorefinery developments. To establish such high solid hydrolysis, a new solid state reactor with static mixing is used. However, concentrations >10% (w/w) cause a rate and yield reduction of enzymatic hydrolysis. By optimizing the synergetic activity of cellulolytic enzymes at solid concentrations of 9%, 17% and 23% (w/w) of crude Organosolv cellulose, glucose concentrations of 57, 113 and 152 g L⁻¹ are reached. However, the glucose yield decreases from 0.81 to 0.72gg⁻¹ at 17% (w/w). Optimal conditions for hydrolysis scale-up under minimal enzyme addition are identified. As result, at 23% (w/w) crude cellulose the glucose yield increases from 0.29 to 0.49gg⁻¹. As proof of its applicability, biobutanol, succinic and itaconic acid are produced with the crude hydrolysate. The potential of the substrate is proven e.g. by a high butanol yield of 0.33gg⁻¹.

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Metadaten
Author:Nils TippkötterORCiD, Anna-Maria Duwe, Sebastian Wiesen, Tim Sieker, Roland UlberORCiD
DOI:https://doi.org/10.1016/j.biortech.2014.06.052
Parent Title (English):Bioresource Technology
Publisher:Elsevier
Place of publication:Amsterdam
Document Type:Article
Language:English
Year of Completion:2014
Volume:167
First Page:447
Last Page:455
Link:https://doi.org/10.1016/j.biortech.2014.06.052
Zugriffsart:campus
Institutes:FH Aachen / Fachbereich Chemie und Biotechnologie
collections:Verlag / Elsevier