Combined supercritical carbon dioxide extraction and chromatography of the algae fatty linoleic and linolenic acid
- A method for the integrated extraction and separation of fatty acids from algae using supercritical CO2 is presented. Desmodesmus obliquus and Chlorella sorokiniana were used as algae. First, a method for chromatographic separation of fatty acids of different degrees of saturation was established and optimized. Then, an integrated method for supercritical extraction was developed for both algal species. It was also verified whether prior cell disruption was beneficial for extraction. In developing the method for chromatographic separation, statistical experimental design was used to determine the optimal parameter settings. The methanol content in the mobile phase proved to be the most important parameter for successful separation of the three unsaturated fatty acids oleic acid, linoleic acid, and linolenic acid. Supercritical extraction with dried algae showed that about four times more fatty acids can be extracted from C. sorokiniana relative to the dry mass used.
Author: | Lena Braun, Simone KrafftORCiD, Nils TippkötterORCiD |
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DOI: | https://doi.org/10.1002/cite.202255308 |
ISSN: | 0009-286X |
ISSN: | 1522-2640 (eISSN) |
Parent Title (English): | Chemie Ingenieur Technik |
Publisher: | Wiley-VCH |
Place of publication: | Weinheim |
Document Type: | Conference: Meeting Abstract |
Language: | English |
Year of Completion: | 2022 |
Date of first Publication: | 2022/08/25 |
Volume: | 94 |
Issue: | 9 |
First Page: | 1304 |
Note: | ProcessNet and DECHEMA‐BioTechNet Jahrestagungen 2022 together with 13th ESBES Symposium 2022, 12. - 15. September 2022, Eurogress Aachen |
Link: | https://doi.org/10.1002/cite.202255308 |
Zugriffsart: | campus |
Institutes: | FH Aachen / Fachbereich Chemie und Biotechnologie |