New Approach for Enzymatic Hydrolysis of Lignocellulose with Selective Diffusion Separation of the Monosaccharide Products
- Enzymatic hydrolysis of lignocellulosic material plays an important role in the classical biorefinery approach. Apart from the pretreatment of the raw material, hydrolysis is the basis for the conversion of the cellulose and hemicellulose fraction into fermentable sugars. After hydrolysis, usually a solid-liquid separation takes place, in order to separate the residual plant material from the sugar-rich fraction, which can be subsequently used in a fermentation step. In order to factor out the separation step, the usage of in alginate immobilized crude cellulose fiber beads (CFBs) were evaluated. Pretreated cellulose fibers are incorporated in an alginate matrix together with the relevant enzymes. In doing so, sugars diffuse trough the alginate matrix, allowing a simplified delivery into the surrounding fluid. This again reduces product inhibition of the glucose on the enzyme catalysts. By means of standardized bead production the hydrolysis in lab scale was possible. First results show that liberation of glucose and xylose is possible, allowing a maximum total sugar yield of 75 %.
Author: | Jasmine Roth, Nils TippkötterORCiD |
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DOI: | https://doi.org/10.1002/cite.201650301 |
ISSN: | 0009-286X |
ISSN: | 1522-2640 (eISSN) |
Parent Title (German): | Chemie Ingenieur Technik |
Publisher: | Wiley-VCH |
Place of publication: | Weinheim |
Document Type: | Conference: Meeting Abstract |
Language: | English |
Year of Completion: | 2016 |
Date of first Publication: | 2016/08/29 |
Volume: | 88 |
Issue: | 9 |
First Page: | 1237 |
Note: | ProcessNet-Jahrestagung 2016 und 32. DECHEMA-Jahrestagung der Biotechnologen 2016, 12. - 15. September 2016, Eurogress Aachen |
Link: | https://doi.org/10.1002/cite.201650301 |
Zugriffsart: | campus |
Institutes: | FH Aachen / Fachbereich Chemie und Biotechnologie |
collections: | Verlag / Wiley-VCH |