Filtern
Erscheinungsjahr
- 2012 (314) (entfernen)
Institut
- Fachbereich Medizintechnik und Technomathematik (70)
- Fachbereich Wirtschaftswissenschaften (42)
- Fachbereich Chemie und Biotechnologie (39)
- Fachbereich Elektrotechnik und Informationstechnik (38)
- Fachbereich Bauingenieurwesen (30)
- INB - Institut für Nano- und Biotechnologien (30)
- IfB - Institut für Bioengineering (29)
- Fachbereich Maschinenbau und Mechatronik (27)
- Fachbereich Energietechnik (20)
- Solar-Institut Jülich (15)
Volltext vorhanden
- nein (314) (entfernen)
Dokumenttyp
- Wissenschaftlicher Artikel (129)
- Konferenzveröffentlichung (81)
- Teil eines Buches (Kapitel) (36)
- Buch (Monographie) (26)
- Konferenz: Meeting Abstract (17)
- Patent (12)
- Dissertation (7)
- Bericht (3)
- Habilitation (1)
- Sonstiges (1)
Schlagworte
- (Bio)degradation (1)
- 3. EU Legislativpaket (1)
- 802.15.4 (1)
- Acceleration (1)
- Adsorption (1)
- Afterload (1)
- Alginate beads (1)
- Anastomotic leakage (1)
- Autolysis (1)
- Avalanche (1)
With a steady increase of regulatory requirements for business processes, automation support of compliance management is a field garnering increasing attention in Information Systems research. Several approaches have been developed to support compliance checking of process models. One major challenge for such approaches is their ability to handle different modeling techniques and compliance rules in order to enable widespread adoption and application. Applying a structured literature search strategy, we reflect and discuss compliance-checking approaches in order to provide an insight into their generalizability and evaluation. The results imply that current approaches mainly focus on special modeling techniques and/or a restricted set of types of compliance rules. Most approaches abstain from real-world evaluation which raises the question of their practical applicability. Referring to the search results, we propose a roadmap for further research in model-based business process compliance checking.
Background
Minor changes in protein structure induced by small organic and inorganic molecules can result in significant metabolic effects. The effects can be even more profound if the molecular players are chemically active and present in the cell in considerable amounts. The aim of our study was to investigate effects of a nitric oxide donor (spermine NONOate), ATP and sodium/potassium environment on the dynamics of thermal unfolding of human hemoglobin (Hb). The effect of these molecules was examined by means of circular dichroism spectrometry (CD) in the temperature range between 25°C and 70°C. The alpha-helical content of buffered hemoglobin samples (0.1 mg/ml) was estimated via ellipticity change measurements at a heating rate of 1°C/min.
Results
Major results were:
1) spermine NONOate persistently decreased the hemoglobin unfolding temperature T u irrespectively of the Na + /K + environment,
2) ATP instead increased the unfolding temperature by 3°C in both sodium-based and potassium-based buffers and
3) mutual effects of ATP and NO were strongly influenced by particular buffer ionic compositions. Moreover, the presence of potassium facilitated a partial unfolding of alpha-helical structures even at room temperature.
Conclusion
The obtained data might shed more light on molecular mechanisms and biophysics involved in the regulation of protein activity by small solutes in the cell.