Refine
Year of publication
Document Type
- Article (3226) (remove)
Language
- English (3226) (remove)
Keywords
- Einspielen <Werkstoff> (7)
- avalanche (5)
- Earthquake (4)
- FEM (4)
- Finite-Elemente-Methode (4)
- LAPS (4)
- biosensors (4)
- field-effect sensor (4)
- frequency mixing magnetic detection (4)
- CellDrum (3)
- Heparin (3)
- Label-free detection (3)
- additive manufacturing (3)
- capacitive field-effect sensor (3)
- hydrogen peroxide (3)
- magnetic nanoparticles (3)
- shakedown analysis (3)
- snow (3)
- tobacco mosaic virus (TMV) (3)
- Acyl-amino acids (2)
Institute
- Fachbereich Medizintechnik und Technomathematik (1343)
- INB - Institut für Nano- und Biotechnologien (501)
- Fachbereich Chemie und Biotechnologie (466)
- IfB - Institut für Bioengineering (408)
- Fachbereich Elektrotechnik und Informationstechnik (401)
- Fachbereich Energietechnik (360)
- Fachbereich Luft- und Raumfahrttechnik (247)
- Fachbereich Maschinenbau und Mechatronik (147)
- Fachbereich Wirtschaftswissenschaften (106)
- Fachbereich Bauingenieurwesen (68)
- Solar-Institut Jülich (43)
- ECSM European Center for Sustainable Mobility (27)
- Sonstiges (21)
- Institut fuer Angewandte Polymerchemie (20)
- Freshman Institute (17)
- Nowum-Energy (16)
- MASKOR Institut für Mobile Autonome Systeme und Kognitive Robotik (15)
- Fachbereich Gestaltung (12)
- Fachbereich Architektur (9)
- ZHQ - Bereich Hochschuldidaktik und Evaluation (5)
To gain insight on chemical sterilization processes, the influence of temperature (up to 70 °C), intense green light, and hydrogen peroxide (H₂O₂) concentration (up to 30% in aqueous solution) on microbial spore inactivation is evaluated by in-situ Raman spectroscopy with an optical trap. Bacillus atrophaeus is utilized as a model organism. Individual spores are isolated and their chemical makeup is monitored under dynamically changing conditions (temperature, light, and H₂O₂ concentration) to mimic industrially relevant process parameters for sterilization in the field of aseptic food processing. While isolated spores in water are highly stable, even at elevated temperatures of 70 °C, exposure to H₂O₂ leads to a loss of spore integrity characterized by the release of the key spore biomarker dipicolinic acid (DPA) in a concentration-dependent manner, which indicates damage to the inner membrane of the spore. Intensive light or heat, both of which accelerate the decomposition of H₂O₂ into reactive oxygen species (ROS), drastically shorten the spore lifetime, suggesting the formation of ROS as a rate-limiting step during sterilization. It is concluded that Raman spectroscopy can deliver mechanistic insight into the mode of action of H₂O₂-based sterilization and reveal the individual contributions of different sterilization methods acting in tandem.