Refine
Year of publication
- 2024 (25) (remove)
Document Type
- Article (16)
- Conference Proceeding (5)
- Book (2)
- Preprint (1)
- Working Paper (1)
Language
- English (25) (remove)
Keywords
- Hot S-parameter (2)
- 1P hub loads (1)
- 3-D printing (1)
- Acyl-amino acids (1)
- Acylation (1)
- Aeroelasticity (1)
- Ageing (1)
- Aminoacylase (1)
- Anatomy (1)
- Biocatalysis (1)
- Biosurfactants (1)
- Capacitive model (1)
- Chaperone (1)
- Circuit simulation (1)
- Complex-valued eigenvalues (1)
- Constitutive model (1)
- Damage mechanics theory (1)
- Discontinuous fractures (1)
- Eigenvalue trajectories (1)
- Enzyme coverage (1)
Institute
- Fachbereich Medizintechnik und Technomathematik (13)
- IfB - Institut für Bioengineering (7)
- INB - Institut für Nano- und Biotechnologien (5)
- Fachbereich Elektrotechnik und Informationstechnik (3)
- Fachbereich Chemie und Biotechnologie (2)
- Fachbereich Luft- und Raumfahrttechnik (2)
- ECSM European Center for Sustainable Mobility (1)
- Fachbereich Wirtschaftswissenschaften (1)
- Institut fuer Angewandte Polymerchemie (1)
Electrolyte-insulator-semiconductor capacitors (EISCAP) belong to field-effect sensors having an attractive transducer architecture for constructing various biochemical sensors. In this study, a capacitive model of enzyme-modified EISCAPs has been developed and the impact of the surface coverage of immobilized enzymes on its capacitance-voltage and constant-capacitance characteristics was studied theoretically and experimentally. The used multicell arrangement enables a multiplexed electrochemical characterization of up to sixteen EISCAPs. Different enzyme coverages have been achieved by means of parallel electrical connection of bare and enzyme-covered single EISCAPs in diverse combinations. As predicted by the model, with increasing the enzyme coverage, both the shift of capacitance-voltage curves and the amplitude of the constant-capacitance signal increase, resulting in an enhancement of analyte sensitivity of the EISCAP biosensor. In addition, the capability of the multicell arrangement with multi-enzyme covered EISCAPs for sequentially detecting multianalytes (penicillin and urea) utilizing the enzymes penicillinase and urease has been experimentally demonstrated and discussed.