Refine
Year of publication
- 2022 (13) (remove)
Document Type
- Article (13) (remove)
Language
- English (13) (remove)
Keywords
- biosensors (2)
- Alkalihalobacillus okhensis (1)
- aminooctanethiol (1)
- aseptic parameters (1)
- atomic layer deposition (1)
- bi-enzyme biosensor (1)
- biocompatible materials (1)
- biodegradable electronic devices (1)
- capacitive field-effect sensor (1)
- capacitive field-effect sensors (1)
- capacitive model (1)
- carbon electrodes (1)
- detergent protease (1)
- electrolyte-insulator-semiconductor capacitors (1)
- enzyme-logic gate (1)
- field-effect sensor (1)
- frequency mixing magnetic detection (1)
- gaseous hydrogen peroxide (1)
- glucose (1)
- gold nanoparticles (1)
Institute
- INB - Institut für Nano- und Biotechnologien (13) (remove)
An improved and convenient ninhydrin assay for aminoacylase activity measurements was developed using the commercial EZ Nin™ reagent. Alternative reagents from literature were also evaluated and compared. The addition of DMSO to the reagent enhanced the solubility of Ruhemann's purple (RP). Furthermore, we found that the use of a basic, aqueous buffer enhances stability of RP. An acidic protocol for the quantification of lysine was developed by addition of glacial acetic acid. The assay allows for parallel processing in a 96-well format with measurements microtiter plates.